Journal: Reproductive Medicine and Biology

Article Title: Exosomal miR ‐203a‐3p Enhances Endometrial Receptivity by Upregulating E‐Cadherin Expression Through the Direct Targeting of SNAI1 in Endometrial Epithelial Cells

doi: 10.1002/rmb2.12689

Figure Lengend Snippet: Effect of miR‐203a‐3p on the regulation of E‐cadherin in endometrial epithelial cells. (A) CDH1 mRNA expression in AN3‐CA and RL95‐2 cells was analyzed using qRT‐PCR. (B) E‐cadherin protein expression in AN3‐CA and RL95‐2 cells was analyzed using western blot. (C) CDH1 mRNA expression in AN3‐CA cells treated with exosomes derived from AN3‐CA or RL95‐2 cells was analyzed using qRT‐PCR. (D) E‐cadherin protein expression in AN3‐CA cells treated with exosomes derived from AN3‐CA or RL95‐2 cells was analyzed using western blot. (E) miR‐203a‐3p expression in AN3‐CA cells transfected with miR‐203a‐3p mimics was analyzed using qRT‐PCR. (F) CDH1 mRNA expression in AN3‐CA cells overexpressing miR‐203a‐3p mimics was analyzed using qRT‐PCR. (G) E‐cadherin protein expression in AN3‐CA cells overexpressing miR‐203a‐3p mimics was analyzed using western blot. (H) E‐cadherin protein localization and expression in AN3‐CA cells overexpressing miR‐203a‐3p mimics were analyzed using immunofluorescence staining. Images were acquired at a magnification of 600×. Scale bar represents 10 μm. (I) Spheroid attachment rate analysis in AN3‐CA cells overexpressing miR‐203a‐3p mimics. The data represent the mean ± SEM from three independent experiments ( n = 3). Statistical significance was assessed using Student's t ‐test (A, C, E, F, I). * p < 0.05; ** p < 0.01.

Article Snippet: We obtained human endometrial epithelial cells (AN3‐CA, RL95‐2) from the American Type Culture Collection (Manassas, VA, USA), and human choriocarcinoma JAr cells from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Derivative Assay, Transfection, Immunofluorescence, Staining

Journal: Reproductive Medicine and Biology

Article Title: Exosomal miR ‐203a‐3p Enhances Endometrial Receptivity by Upregulating E‐Cadherin Expression Through the Direct Targeting of SNAI1 in Endometrial Epithelial Cells

doi: 10.1002/rmb2.12689

Figure Lengend Snippet: Proposed mechanism for enhancing endometrial receptivity through miR‐203a‐3p‐mediated regulation of the SNAIL/E‐cadherin pathway. EEC, endometrial epithelial cell; OE, overexpressing.

Article Snippet: We obtained human endometrial epithelial cells (AN3‐CA, RL95‐2) from the American Type Culture Collection (Manassas, VA, USA), and human choriocarcinoma JAr cells from the Korean Cell Line Bank (Seoul, Korea).

Techniques: